SrasV1, Main, Exploration, bibRecord, 005201

Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.

Identifieur interne : 005201 ( Main/Exploration ); précédent : 005200; suivant : 005202

Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.

Auteurs : Wenbing Chen [République populaire de Chine] ; Shousong Li ; Biying Shao ; Teng Zheng ; Shuxun Jiang ; Xiaorong Huang ; Kaizhen Cai ; Zhideng Zhang

Source :

Genomics, proteomics & bioinformatics [ 1672-0229 ] ; 2004.

RBID : pubmed:15629044

Descripteurs français

KwdFr :
- ADN complémentaire (génétique), Amorces ADN, Clonage moléculaire, Données de séquences moléculaires, Réaction de polymérisation en chaîne (), Séquence nucléotidique, Virus du SRAS (génétique).
MESH :
- génétique : ADN complémentaire, Virus du SRAS.
- Amorces ADN, Clonage moléculaire, Données de séquences moléculaires, Réaction de polymérisation en chaîne, Séquence nucléotidique.

English descriptors

KwdEn :
- Base Sequence, Cloning, Molecular, DNA Primers, DNA, Complementary (genetics), Molecular Sequence Data, Polymerase Chain Reaction (methods), SARS Virus (genetics).
MESH :
- chemical , genetics : DNA, Complementary.
- chemical : DNA Primers.
- genetics : SARS Virus.
- methods : Polymerase Chain Reaction.
- Base Sequence, Cloning, Molecular, Molecular Sequence Data.

Abstract

The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully.

DOI: 10.1016/s1672-0229(04)02008-x
PubMed: 15629044

Affiliations:

Le document en format XML

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<term>Molecular Sequence Data</term>
<term>Polymerase Chain Reaction (methods)</term>
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<term>Séquence nucléotidique</term>
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<term>Molecular Sequence Data</term>
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<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
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<front><div type="abstract" xml:lang="en">The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully.</div>
</front>
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<name sortKey="Zhang, Zhideng" sort="Zhang, Zhideng" uniqKey="Zhang Z" first="Zhideng" last="Zhang">Zhideng Zhang</name>
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Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.

Preliminary study on the detection of the SARS-CoV specific target cDNA fragments by multiplex PCR.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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